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3D单分子荧光成像系统 |
abbelight SAFe360是一款基于单分子定位的显微成像（SMLM）的3D单分子成像系统，它独有的DAISY技术整合了散光技术和超临界角光技术，能够极大的提高定位精度，达到**的xyz同时15nm的定位精度，可以提供清晰度**的三维的亚细胞结构图像，**同时四色成像观测，可以实时研究不同的结构功能蛋白的共定位信息，在单分子水平研究分子动力学反应以及细胞间的相互作用等。
3D单分子荧光成像系统-SAFe 360
设备参数 |
+ 成像模式：PALM、STORM、PAINT、smFRET 、SPT
+ 光源模式：Epi、TIRF、HILO
+ **分辨率：15 nm的XYZ轴分辨率
+ 超大视野：200 × 200 μm2的视野
+ 一次可同时采集1.2 μm深度图像信息
+ **图像深度：10 μm
+ 实时漂移矫正
+ **四色同时成像
+ 活细胞成像模式
加装 | TIRF PALM STORM SPT smFRET ...... | 兼容 | Confocal Spinning-Desk Widefield SIM STED |
Now We See......
3D线粒体结构 | 核孔复合物 |
| 老鼠海马神经元 | 微管蛋白网络 |
配套试剂
Smart kit | Compatible dyes |
• 10 doses per box • 200 μL per dose • 30 sec prepartion • 2 months in a fridge • 2 weeks on sample | • Atto 488， WGA-AF®488 • AF®532， CF®532， Cy3b • AF®555， AF®594， CF®555， AF®568， CF®568， Cy5， MemBriteTM 568， TMR • AF®647， CF®647， AF®680， CF®680， MemBriteTM 640， Actin-stain 670， SiR647 |
发表文献列表
[1] Cabriel， Clément， et al. "Combining 3D single molecule localization strategies for reproducible bioimaging." Nature communications 10.1 (2019): 1980.
[2] Woodhams， Stephen G.， et al. "Cell type–specific super-resolution imaging reveals an increase in calcium-permeable AMPA receptors at spinal peptidergic terminals as an anatomical correlate of inflammatory pain." Pain 160.11 (2019): 2641-2650.
[3] Belkahla， Hanen， et al. "Carbon dots， a powerful non-toxic support for bioimaging by fluorescence nanoscopy and eradication of bacteria by photothermia." Nanoscale Advances (2019).
[4] Denis， Kevin， et al. "Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease." Nature microbiology 4.6 (2019): 972.
[5] Szabo， Quentin， et al. "TADs are 3D structural units of higher-order chromosome organization in Drosophila." Science advances 4.2 (2018): eaar8082.
[6] Boudjemaa， Rym， et al. "Impact of bacterial membrane fatty acid composition on the failure of daptomycin to kill Staphylococcus aureus." Antimicrobial agents and chemotherapy 62.7 (2018): e00023-18.
[7] Culley， Sian， et al. "Quantitative mapping and minimization of super-resolution optical imaging artifacts." Nature methods 15.4 (2018): 263.
[8] Berger， Stephen L.， et al. "Localized myosin II activity regulates assembly and plasticity of the axon initial segment." Neuron 97.3 (2018): 555-570.
[9] Cabriel， Clément， et al. "Aberration-accounting calibration for 3D single-molecule localization microscopy." Optics letters 43.2 (2018): 174-177.
[10] Bouissou， Ana?s， et al. "Podosome force generation machinery: a local balance between protrusion at the core and traction at the ring." ACS nano 11.4 (2017): 4028-4040.
[11] Sellés， Julien， et al. "Nuclear pore complex plasticity during developmental process as revealed by super-resolution microscopy." Scientific reports 7.1 (2017): 14732.
[12] Bourg， Nicolas， et al. "Direct optical nanoscopy with axially localized detection." Nature Photonics 9.9 (2015): 587.
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